Protein dynamics and interactions research group

Group leader

• György Vámosi, PhD, senior scientist

Staff

• Gábor Mocsár, PhD, research fellow
• Éva Hegedüs, PhD, research fellow
• Julianna Volkó, PhD, research fellow
• Ádám Kenesei, MSc, junior research fellow
• Lina Fadel (Ms), PharmD, PhD student
• Pialy Sen (Ms), MSc, PhD student
• Anass Benziane (Mr), MSc, PhD student
• Edina Nagy, technician

Former members of the lab

• Nikoletta Szalóki, PhD, PhD student, research fellow
• István Hajdu, PhD, PhD student
• Veronika Sebestyén, MD, diploma student
• Anna Madarász, diploma student
• Anita Csizmarik, MSc, diploma student
• Katalin Veres, MSc, diploma student
• Kitti Balogh, MSc, diploma student
• Balázs Kreith, MSc, diploma student
• Mariann Parag, MSc, diploma student
• Éva Nagy, PhD student
• Balázs Bravics, PhD student
• Malte Renz, MD, PhD, postdoctoral fellow
• Attila Horváth, MSc, diploma student
• Gergely Molnár, MSc, diploma student
• Enikő Menczel, MSc, diploma student
• Tibor Szekeres, MSc, diploma student
• Tamás Domoszlai, PhD, diploma student
• Rita Utasi-Szabó, technician
• Áron Zubánovics-Nagy, diploma student
• Bianka Csaholczi, research student
• Bálint Rehó, MD, PhD student (correspondent)

Background

Representative publications

• Vámosi G, Baudendistel N, von der Lieth CW, Szalóki N, Mocsár G, Müller G, Brázda P, Waldeck W, Damjanovich S, Langowski J, Tóth K: Conformation of the c-Fos / c-Jun complex in vivo: a combined FRET, FCCS and MD-modeling study. Biophys J. 94(7):2859-68, 2008
• Bodnár A, Nizsalóczki E, Mocsár G, Szalóki N, Waldmann TA, Damjanovich S, Vámosi G: A biophysical approach to IL-2 and IL-15 receptor function: localization, conformation and interactions. Immunol Lett. 116(2):117-25, 2008.
• Vámosi G*, Bodnár A*, Vereb G, Jenei A, Goldman CK, Dubois S, Langowski J, Tóth K, Mátyus L, Szöllősi J, Waldmann TA, Damjanovich S: Interleukin-2 and -15 receptor alpha subunits are co-expressed in a supramolecular receptor cluster in lipid rafts of T-cells. Proc Natl Acad Sci U S A. 101(30):11082-7, 2004.
• Panyi G.*, Vámosi G*, Bacsó Z*, Bagdány M, Bodnár A, Varga Z, Gáspár R, Mátyus L, Damjanovich S: Kv1.3 potassium channels are localized in the immunological synapse formed between cytotoxic and target cells. Proc Natl Acad Sci U S A 101(5): 1285-90, 2004.
• Brazda P, Szekeres T, Bravics B, Tóth K, Vámosi G*, Nagy L*: Live-cell fluorescence correlation spectroscopy dissects the role of coregulator exchange and chromatin binding in retinoic acid receptor mobility. J Cell Sci. 2011 Nov 1;124(Pt 21):3631-42.

Recent publications

• Vámosi G, Mücke N, Müller G, Krieger JW, Curth U, Langowski J, Tóth K: EGFP oligomers as natural fluorescence and hydrodynamic standards. Sci Rep. 2016 13;6:33022.
• Mocsár G, Volkó J, Rönnlund D, Widengren J, Nagy P, Szöllősi J, Tóth K, Goldman CK, Damjanovich S, Waldmann TA, Bodnár A, Vámosi G: MHC I Expression Regulates Co-clustering and Mobility of Interleukin-2 and -15 Receptors in T Cells. Biophys J. 2016 111:100-12.
• Szalóki N, Doan-Xuan QM, Szöllősi J, Tóth K, Vámosi G*, Bacsó Z*:1.  High throughput FRET analysis of protein-protein interactions by slide-based imaging laser scanning cytometry. Cytometry A. 2013 83:818-29.
• Brazda P, Krieger J, Daniel B, Jonas D, Szekeres T, Langowski J, Tóth K, Nagy L*, Vámosi G*: 1.      Ligand binding shifts highly mobile retinoid X receptor to the chromatin-bound state in a coactivator-dependent manner, as revealed by single-cell imaging. Mol Cell Biol. 2014 34:1234-45.
• Renz M, Daniels BR, Vámosi G, Arias IM, Lippincott-Schwartz J: 1.       Plasticity of the asialoglycoprotein receptor deciphered by ensemble FRET imaging and single-molecule counting PALM imaging. Proc Natl Acad Sci U S A. 2012 109:E2989-97.
• Mocsár G, Kreith B, Buchholz J, Krieger JW, Langowski J, Vámosi G: Multiplexed multiple-tau auto- and cross-correlators on a single field programmable gate array. Rev Sci Instrum. 2012 83:046101.

Figure 1: Possible cytokine dependent arrangements of the IL-2/IL-15 receptor complex together with MHC I and MHC II based on FRET and FCCS data

Figure 2: Different states of RAR as detected by FCS. Left, the majority of the receptors (NR) in their unliganded state are present in a monomeric/dimeric form or as small complexes diffusing freely. Right, the fraction of the chromatin-bound slow population is increased when the receptor binds an agonist.

Figure 3: MD modeling structures of the Jun-Fos complex compatible with FRET results.